Cell and Tissue Journal

Abstract Aim: The aim of the present study was to investigate the effects of royal jelly (RJ) and vitamin C (vit C) against PHZ-induced nephropathy in mice.
Material and Methods: Adult male mice were randomly divided into eight eight-membered groups. The PHZ was administered to four groups of mice at a dose of 60 mg/kg per 48 hours intraperitoneally for 35 days. Three of these groups received vit C (250 mg/kg per day) intraperitoneally, RJ (100 mg/kg per day) orally and vit C+RJ with same doses, four hours before PHZ administration, respectively. A vehicle-treated control group and vit C, RJ and vit C+RJ control groups were also included. Serum and renal tissue samples were collected 24 hours after the last treatment and subjected to biochemical and histological examinations, respectively. The data were analyzed by one-way analysis of variance followed by Duncan test for post hoc comparisons.
Results: The PHZ treatment caused a significant (P < 0.05) increased malondealdehyde concentration in serum, but decreased total antioxidant capacity of it. Moreover, PHZ significantly (p < 0.05) increased luminal diameter of proximal convoluted tubule, while decreased (p < 0.05) their epithelial cells height. Considerably, co-treatment of vit C and RJ improved all changes in the above-mentioned parameters.
Conclusion: The Vit C and RJ can minimize PHZ-induced nephropathy in mouse through oxidative reactions inhibition.

Abstract Aim: Para-Nonylphenol is an environmental pollutant which can generate free radicals causing tissue damage. The aim of this study was to investigate the effect of vitamin E as a strong antioxidant on kidney tissue in rats treated with para-Nonylphenolusing’stereological technique.
Material and Methods: Twenty fourmaleWistar rats with the mean body weight of 198±20 g were randomly divided into 4 groups (n=6), control, vitamin E (100mg/kg/day), Para-Nonylphenol (250mg/kg/day) and Para-Nonylphenol+ vitamin E, and treated orally for 56 days. Finally, the rats were weighted and their right kidneys were taken out, fixed, sectioned, processed and stained using Heidenhain̕& azan method. The kidney tissue was then studied using stereological methods. Data were analyzed using one way ANOVA and means difference was considered significant at p < 0.05.
Results: The mean kidney weight, the total volume of kidney, the volume of cortex and medulla, the volume of proximal and distal tubule lumen, interstitial tissue and the volume of glomerulus and tuft significantly increased in Para-Nonylphenol group compared to the control ones, while the volume of proximal and distal tubules epithelium and the volume of Bowman’s space reduced significantly.
Conclusion: Vitamin E could compensate many adverse effects of Para-Nonylphenol on the kidney tissue. Consequently, vitamin E could be used as a potential antioxidant (supplement) against NP intoxication.