Protective Effect of Nano-Zinc Oxide on Histological Parameters of Testis Following Doxorubicin Treatment
Volume 4, Issue 2, Autumn 2013, Pages 159-167
https://doi.org/10.52547/JCT.4.2.159
Abstract Aim: Doxorubicin (DOX) is one of the most widely used antineoplasic drugs although it is toxic for different parts of the body like reproductive organs. The aim of this study was to investigate the protective effect of nano-Zinc oxide on doxorubicin-induced reproductive toxicity.
Material and Methods: Adult male Wistar rats were divided in four groups including one control and three experimental groups. The control group received Saline (i.p). The experimental groups received Doxorubicin (6 mg/kg), nano-Zinc oxide (5 mg/kg) and Doxorubicin following nano-Zinc oxide respectively. Treatment was performed for 3 days. 28 days after treatment, histological changes in testis were assessed.
Results: The DOX group showed disintegrated germinal epithelium, hypoplasia and deformation of cells, and increased interstitial space. Spermatocyte, spermatid and spermatozoid numbers as well as Leydig cell numbers were reduced. Also Dox increased the number of sloughing tubules while it decreased tubule differentiation index (TDI) and spermiation index (SPI). Nano-Zinc oxide treatment resulted in significant improvement of DOX-induced disorders.
Conclusion: The protective effect of nano-Zinc Oxide is illuminated in DOX-induced male reproductive system toxicity.