Cell and Tissue Journal

Abstract Aim: The aim of this experimental study was to investigate the effective infertility factors of physoaline compounds on the adult reproductive system of Balb/C adult female.
Material and Methods: The above experiment was performed by preparing the aqueous extract of the plant and selecting three doses of 7.5g/kg,9g/kg and 15g/kg;Simultaneously with the experimental groups,the distilled water sham group was injected and the control group was kept intact.Vaginal stromal smear cycles were prepared to examine different stages.One hour after the last injection,the mice were given blood samples and then killed by chloroform and their uterus and ovaries were prepared for histological studies.
Results: Statistical studies have shown that injecting 7.5g/kg of extract reduces estrogen and restores 33% of fertility in mice. Also, at a dose of 9 g/kg, the mice were initially infertile, but one month after the last injection, 16.5% of the mice showed a return to fertility and a significant increase in progesterone.
Conclusion: According to the results, it is probably because physalines have a pseudo-steroidal structure. The receptor is occupied by physaline and can cause abnormalities in the growth and development of oocytes, uterine tissue cells and ovaries, and infertility.
 

Abstract Aim: Diabetes as a metabolic disorder affects different tissues and organs, among them the female reproductive tract such as uterus may be a vulnerable organ. This study was undertaken to study the effects of hyperglycemia and insulin therapy on uterus structure, estrous cycle and serum levels of pituitary and ovarian hormones in Wistar rats.
Material  and methods: Twenty-four female Wistar rats (180-200g) were randomly divided in: control, sham (Citrate buffer i.p), hyperglycemic (Streptozotocin; 60 mg/kg, i.p) and hyperglycemic+insulin (insulin 20 IU/Kg/day; s.c). At the end of experiment (36 Days) and in diestrus stage, all rats were weighted and blood samples were collected. Thereafter, their right uterus horn was taken out, weighted and then the middle third part of uterine horn was sampled and fixed (Bouein). After histological preparation, the histological and histomorphometrical examination of uterus was performed.
Results: Although in comparison with control and hyperglycemic+insulin groups, in hyperglycemic rats body weight, uterus weight and volume, endometrium and myometrium volume were dramatically decreased (p < 0.001) and the serum level of progesterone significantly increased (p < 0.001), vascular dilation and infiltrating cells resembling plasma cell was also observed. No significant difference was seen between control and insulin treated group for the above parameters.
Conclusion: Insulin deficiency, oxidative stress and alteration in the HPG axis which occurs in hyperglycemic condition, may affect the uterus structure. The observed structural changes might well play a significant role in the reproductive difficulties observed during hyperglycemia