Cell and Tissue Journal

Abstract Aim: The aim of this study was to investigate the protective effect of Nigella sativa oil (NSO),as an antioxidant, on the testicular tissue in mice treated with bisphenol A.
Material and Methods: In this study, 24 adult male mice NMRI were divided into four groups (n=6): control, bisphenol A (200 mg/kg/day), nigella sativa oil (5 ml/kg/day) and bisphenol A + nigella sativa oil. 34 days after oral treatment, the right testis was taken out, fixed and processed followed by Heidenhain's Azan staining. The morphometric parameters of the testicular tissue were evaluated using stereological methods.
Results: The mean volume of seminiferous tubules and it's height of the germinal epithelium,  the  mean diameter and thickness of the basement membrane, the mean number of spermatocytes, round and long spermatids, spermatogonia and sertoli cells significantly reduced in the bisphenol A group compared to the controls (p < 0.05). The mean volume of seminiferous tubules and the mean number of Sertoli cells significantly increased in the bisphenol A + nigella sativa oil group to the control level,  The mean number of spermatocytes and round and long spermatids also showed a significant increase in this group compared to the bisphenol A group (p < 0.05).
Conclusion: Co-administration of nigella sativa oil with bisphenol A can prevent the adverse effects of bisphenol A on the testicular tissue in adult mice.
 
 

Abstract Aim: The aim of the present study was to stereological evaluate the protective effect of green tea extract on the morphological changes of seminiferous tubules following sodium arsenite exposure in mice.
Material and Methods: 24 adult male NMRI mice were randomly divided into four groups including control, sodium arsenite (5mg/kg/day), green tea extract (100 mg/kg/day) and finally sodium arsenite+green tea extract. Mice were (orally) treated for 34 days. At the end, mice were sacrificed and their right testis were taken out, fixed, processed and stained with heidenhain' azan method. The morphological and changes of testicular tissue was examined using stereological methods. Data were analyzed using one way ANOVA and means difference was considered significant at p < 0.05.
Results: Sodium arsenite treatment caused a significant decrease in the mean seminiferous tubules diameter (p < 0.008), germinal epithelium height (p < 0.001) and the basement membrane thickness (p < 0.011) as well as in the number of spermatocyte (p < 0.003), round spematid (p < 0.028), long spermatid (p < 0.011) and sertoli (p < 0.03) cells compared to the control group. The above changes increased to the control level in sodium arsenite+green tea exteract group.
Conclusions: The results indicate that green tea extract may be useful in reducing the toxic effects induced by sodium arsenite.

Abstract Aim: The aim of this study was to evaluate histometrical and stereological properties of the different tissue layers in male and female goats.
Material and Methods: To study the microscopic structure of the goat esophagus by stereological method, the eight healthy adult goats from both sexes were selected and the cervical region was dissected and esophagus removed. The obtained samples were fixed in 10% buffered formalin solution and then prepared tissue sections, stained with hematoxylin and eosin and studied using light microscopy.
Results: Results showed that there are significant variation in micrometry thicknesses of the submucosal and muscular layer at the cervical and thoracic regions (p < /em><0.05). The stereological results showed that mean volume of the esophageal muscular layer was significantly greater in male than female, but in other layers significant differences between both sexes was not observed (p < /em>>0.05).
Conclusion: Although morphometrical results showed that there are significant variations in different layers of the esophagus tissue, but stereological results showed no significant difference.
 

Abstract Aim: Para-Nonylphenol is an environmental pollutant which can generate free radicals causing tissue damage. The aim of this study was to investigate the effect of vitamin E as a strong antioxidant on kidney tissue in rats treated with para-Nonylphenolusing’stereological technique.
Material and Methods: Twenty fourmaleWistar rats with the mean body weight of 198±20 g were randomly divided into 4 groups (n=6), control, vitamin E (100mg/kg/day), Para-Nonylphenol (250mg/kg/day) and Para-Nonylphenol+ vitamin E, and treated orally for 56 days. Finally, the rats were weighted and their right kidneys were taken out, fixed, sectioned, processed and stained using Heidenhain̕& azan method. The kidney tissue was then studied using stereological methods. Data were analyzed using one way ANOVA and means difference was considered significant at p < 0.05.
Results: The mean kidney weight, the total volume of kidney, the volume of cortex and medulla, the volume of proximal and distal tubule lumen, interstitial tissue and the volume of glomerulus and tuft significantly increased in Para-Nonylphenol group compared to the control ones, while the volume of proximal and distal tubules epithelium and the volume of Bowman’s space reduced significantly.
Conclusion: Vitamin E could compensate many adverse effects of Para-Nonylphenol on the kidney tissue. Consequently, vitamin E could be used as a potential antioxidant (supplement) against NP intoxication.