Investigation of Antioxidant properties of Ocimum basilicum and Impatiens walleriana and their cytotoxic effect on gastric cancer AGS cell line
Volume 9, Issue 4, Winter 2019, Pages 378-387
https://doi.org/10.52547/JCT.9.4.378
P Hanachi, Sh Salehizadeh, K Kiarostami, R Ramezani
Abstract Aim:This study was performed to evaluate the cytotoxic effect of Ocimum basilicum and Impatiens walleriana aqueous extract on the gastric cancer of AGS cell line.
Material and methods: FRAP method was used to measure the antioxidant activity of Ocimum basilicum and Ipatiems walleriana extracts. After preparation of O. basilicum and I. walleriana aqueous extract, we measured the anticancer effect on growth of AGS cancer cells at 24, 48 and 72 hours by MTT.
Results: The highest antioxidant activity of O. basilicum and I. walleriana was measured by FRAP method for water solvent and water bath extraction method, which in O. basilicum was
0.778 ± 0.11 mM and in I.walleriana 1.30 ± 0.03 mM. Based on the obtained data of the MTT test, the highest toxicity of the extract occurs 72 hours after adding the extract to the cells. The lowest amount of IC50 of the aqueous extract of O. basilicum was measured at 0.012 ± 0.002 mg / ml and in I. walleriana extract was 2.87 ± 0.001 mg / ml., which had a significant difference ( p <0.05).
Conclusion: The results of the anticancer study of the aqueous extract of O. basilicum and I. walleriana indicate that these plants have a cytotoxicity effect in the higher concentrations and need a long duration for their effects.