Cell and Tissue Journal

Abstract Aim: The aim of this study was to assess the protective effect of Meristotropis xanthioides extract on ethanol-induced hepatotoxicity.
Material and methods: The total phenol and flavonoid contents of the species were measured by Folin Ciocalteu and AlCl₃, respectively. Male Wistar rats were divided into three groups: group 1 (control), group 2: received 1ml 50 % ethanol, group 3 : received 1ml ethanol (50 %) plus the extract (500 mg/kg), each group consisted of six rats. All treatments were performed by intragastric administration. Silymarin was used as positive control. Biochemical, histological and morphological analyses were used for evaluation of hepatotoxicity.
Results: The extract had high total phenolic and flavonoid contents. Alcohol consumption significantly increased liver/body weight ratio, amounts of tissue’s malondialdehyde (MDA), H₂O₂ and also liver enzymes in blood in comparison to other groups. Histological examination showed that alcohol consumption injures liver tissue intensely. All these alcohol-induced changes were effectively inhibited by treatment with the extract.
Conclusion: This research confirmed that the species can represent the protective role on ethanol-induced hepatotoxicity and suggested its capacity to apply as a new healthcare food and drug supplement.

Abstract Aim: In the present study the effect of oxidative stress resulted from hydrogen peroxide on some secondary metabolites and changes on intra cellular hydrogen peroxide has been explored. Material and methods: To induction of callus Periwinkle leaf was sterilized and put on MS medium. Calluses were treated by different concentrations (1, 5 and 10 µM) of hydrogen peroxide for 6 days. Total alkaloid, phenolic components and felavonoids as well as antioxidant potential on the basis of Iron reduction were determined.  Data were analyzed using one way ANOVA. Results: Addition of hydrogen peroxide resulted in significant (P≤0.05) increase of intra cellular hydrogen peroxide compared to the control. Total alkaloids, flavonoids and phenols content showed that hydrogen peroxide caused a significant increase (P Conclusion: Hydrogen peroxide increased intra cellular levels of hydrogen peroxide. Secondary metabolites such as alkaloids and phenolic compounds and total flavonoid were boosted as well. Therefore tensions may be applied to increase them.