Cell and Tissue Journal

Abstract Aim: Plant production from tissue culture is of high importance from genetic engineering point of view, so in this study, optimization of tissue culture conditions for callus production and plant regeneration of Salsola arbuscula were examined. Material and Methods: Seeds were planted MS medium and after two months, roots, stems (internodes) and leaves explants were transferred to the MS medium with different concentration of 2,4-D and Kinetin hormones for production callus. Plant regeneration was also examined on the various media. Results: Results showed that the best medium to induce callus production were medium of MS + 2,4-D (1 mg/L) + Kin(1 mg/L) and the best explants were roots. So, direct shoot regeneration produced in the medium MS + Kin(1 mg/l) and MS + Kin (0.5 mg/L). Conclusion: Despite production of callus on medium containing only 2,4-D, combined two hormones, auxin and cytokinin, increased the rate of callus production. Also direct shoot regeneration has occurred in medium without auxin. So, production rate of callus and plant regeneration are dependent on amount of external plant growth regulators, and also, amount of external plant growth regulators significantly dependent to genotype and amount of internal plant hormones.

Abstract Aim: In this study, the effects of NaCl on seed germination leaf and stem anatomy and growth parameters were investigated in 10 and 60 days-old of Salsola arbuscula species.
Material and Methods: The species seeds were cultivated in WA (0, 100, 200, 250, 300 mM NaCl), and MS (0, 100, 200, 250, 300, 350 mM NaCl) in vitro media. Then 10 and 60 days-old plants were used for studying seed germination and growth parameters. In addition to leaf anatomy of 60 days-old plants in MS (0, 100, 200, 250, 300, 350 mM NaCl) and after recovery in MS (300, 350, 400 mM NaCl), germination percentages were also investigated.
Results: Results showed decline in germination percentage and ability of recovery in germination percentage. Also growth and anatomical parameters showed wide varieties in 10 and 60 days-old plants with increasing NaCl concentration.
Conclusion: S .arbuscula is a halophytic plant that shows a better growth in low concentration of NaCl than control. Also the species protects its water content along the stress condition on the contrary glocophytes and has germinating ability. These characters help to survival of the species.