The interaction effect of salinity stress and superabsorbent polymer on antioxidant enzyme activities of basil
Volume 9, Issue 3, Winter 2019, Pages 222-237
https://doi.org/10.52547/JCT.9.3.222
S Farsari, M Moghaddam
Abstract Aim: The aim of this study is to evaluate the interaction effect of salinity stress and superabsorbent polymers on physiological traits of basil.
Material and methods: A pot experiment was conducted as factorial based on a completely randomized design with four levels of salinity (0, 40, 80 and 120 mM NaCl in irrigation water) and four levels of superabsorbent polymers included (control, Ackoasorb, Stockosorb and Terracottem). The measured traits were soluble protein, antioxidant enzyme activities, malondialdehyde content and essential oil production.
Results: Antioxidant enzyme activities of catalase, guaiacol peroxidase, ascorbate peroxidase and polyphenol oxidase at first harvesting and high salinity (120 mM) under Ackoasorb, Terracottem, Terracottem and Ackoasorb usage decreased 52.68, 73.1, 68.07 and 75.35%, respectively, and also at second harvest at highest salinity level (80 mM) the activity of these enzymes under Ackoasorb, Terracottem, Ackoasorb, Terracottem and Terracottem decreased 37.6, 62.5, 46.38 43.06 and 38.47%, respectively. With increasing salinity the essential oil production decreased and Tracheotem superabsorbent increase it. At first harvesting, a significant positive correlation was observed between malondialdehyde with guaiacol peroxidase (r = 0.751) and at second harvesting ascorbate peroxidase with protein (r = - 0.753) had a significant negative correlations, moreover, significant positive correlations were found between superoxide dismutase with guaiacol peroxidase (r = 0.848), malondialdehyde with guaiacol peroxidase (r = 0.789) and malondialdehyde with ascorbate peroxidase (r = 0.743).
Conclusion: The results showed that application of superabsorbents under salinity stress conditions could decrease the severity of this stress and thereby cause to decrease the antioxidant enzyme activities and malondialdehyde amount significantly, but increased the soluble protein and essential oil content.
Effect of curcumin on plasma membrane integrity and stress oxidative factors testis and serum in mice treated with cadmium chloride
Volume 9, Issue 1, Summer 2018, Pages 1-11
https://doi.org/10.52547/JCT.9.1.1
sh Chehreii, HR Momeni, Z Atabaki
Abstract Aim: The current research was done to investigate the sperm plasma membrane integrity, oxidative stress factors of sperm and testis in male mice treated with cadmium.
Material and Methods: 24 adult NMRI mice were divided into four groups: 1. Control group 2. Treated with cadmium chloride (5 mg/kg) 3. Treated with Curcumin (100 mg/kg) 4. Treated with curcumin + cadmium chloride. The treatments were performed as a single dose and after 24 hours, epididymal spermatozoa of different groups were used to evaluate the plasma membrane integrity. In addition, the amount of malondialdehyde (MDA) and the anti-oxidant activity of serum and testis were evaluated. Data were analyzed using ANOVA test followed by Turkey’s test (p < 0.05).
Results: findings showed that cadmium chloride caused a significant decrement in plasma membrane integrity compared to the control groups. In addition, cadmium chloride induced a significant increment of MDA in serum and testis and a significant decrement in total antioxidant activity of serum and testis as compared to the group. In curcumin + cadmium chloride- treated group, curcumin could significantly compensate the toxic effect of cadmium on these parameters compared to the cadmium-treated group.
Conclusion: it seems thatcurcumin as a potent antioxidant is able to ameliorate the adverse effects of cadmium chloride on sperm plasma membrane integrity, lipid oxidation and total antioxidant activity of serum and testis.