Effect of Aluminum Toxicity on Inducing of Apoptosis in Cell Suspension of Rice (Oryza sativa) Khazar and Tarom Cultivars
Volume 5, Issue 1, Summer 2014, Pages 1-9
https://doi.org/10.52547/JCT.5.1.1
A N, M M, M Y, MR A
Abstract Aim: In this study the effect of AlCl3 toxicity on apoptosis in cells suspension of two rice cultivars Khazar, Tarom were investigated.
Material and Methods: In present research cells suspension of two rice cultivars (Khazar and Tarom) grew up in liquid Murashig and Skoog medium within a period of 3 weeks. Then cells were treated with different concentrations (0, 40, 80 and 120 µmolL-1) of AlCl3 for 56 hours. Biochemical changes of DNA by gel electrophoresis and TUNEL test and morphological changes by Hoechst and propidium iodide (PI) staining were investigated.
Results: Results of gel electrophoresis and TUNEL test showed DNA damage in these cultivars. Results of Hoechst and PI dying also showed morphological changes such as: condensation of nucleus, membrane damage and shrinkage of cytoplasm.
Conclusion: AlCl3 induced nuclear DNA damage and also morphological change in two cultivars of rice especially Tarom by increasing concentration after 56 hours of treats.