Cell and Tissue Journal

Abstract Aim: This study was done to evaluate the therapeutic effects of Curcuma longa L. rhizome powder and bovine ghee mixture on experimental stomach ulcers in rat.
Material and methods: Stomach ulcer induction was done in 48 food deprived male rats (250-300 gr) using orally Indomethacin (50 mg/kg suspended in 1% carboxymethyl cellulose. Animals divided randomly into five groups. The normal and untreated (Indomethacin) group just received normal saline. The treated groups received different doses of C. longa and bovine ghee (500 and 750 mg C. longa in 10 ml ghee/per kg) for three successive days. On the third day, the rats were killed and their stomachs were removed for histological studies. Stomach ulcers number and length were measured and also ulcer index was calculated.
Results: Results showed that the mixture of C. longa and bovine ghee is significantly caused decreasing gastric ulcer index, inflammatory cells, blood capillaries densities (p < 0.001) and increasing mucosal layer thickness (p < 0.001) and secreted mucus (p < 0.05) in treated groups in comparison with untreated group.
Conclusion: According to the results, the mixture of C. longa and bovine ghee is significantly accelerated healing of experimental stomach ulcers.

Abstract Aim: In this study the synergic effect of Chiton lamyi foot alcoholic extract and decellulation rat brain’s tissue on angiogenesis in hen (chick???) chorioalantoic membrane (CAM) was investigated. Material and Methods: In this study, 70 fertilized eggs of Ross strain were divided into 7 treatments groups in ten repetitions: 1. control, 2. DMSO, 3. decellulation rat brain’s tissue, 4. 10mgμl^-1 Chiton lamyi foot alcoholic extract, 5. 20mgμl^-1 Chiton lamyi foot alcoholic extract, 6. decellulation rat brain’s tissue & 10mgμl^-1 Chiton lamyi foot alcoholic extract and 7. decellulation rat brain’s tissue & 20mgμl^-1 Chiton lamyi foot alcoholic extract. All of eggs were placed in the incubator for eight days and treatments induction was done in 8^th day. Then they photographed using photo steriomicroscope in 12^th day. Blood vessels branches length and number were evaluated by Image J software in treatment sites on chorioalantoic membrane. Data were analyzed using ANOVA, SPSS Test (p < /em><0.05). Results: Results showed that Chiton lamyi foot alcoholic extract, 10 and 20mgμl^-1 Chiton lamyi foot alcoholic extracts with decellulation rat brain’s tissue treatments significantly decreased blood vessels branches length and number in comprising with control and DMSO treatments (p < /em>< 0.05). Conclusion: The Synergic usage of Chiton lamyi foot alcoholic extract and decellulation (acellular) rat brain’s tissue has dose inhibitory effect on hen chorioalantoic membrane (CAM) angiogenesis.  

Abstract Aim: In this study, we have used immunohistochemical studies to examine the effects of maternal hypothyroidism on the expression of laminin in the lung alveoli newborn Wistar rats examined.
Material and methods: in this study we have used female virgin rat, wistar strain. Rats were divided in two experimental groups (hypothyroid and hyperthyroid treated with thyroxin) and one control group (n=12). 14 days before mating in the experimental groups, the rats respectively were exposed to Propylthiouracil (50 mg/L) as well as thyroxin (1 mg/L) and Propylthiouracil (50 mg/L) simultaneously. After 14 days, blood test was taken from mothers and in case of desired changes in hormone levels, rats were allowed to mate. After delivery, the lung of the 10 days old newborns were used for immunohistochemical studies.
Results: There was a significant reduction in laminin expression in hypothyroid group (p= 0.001) where as no significant difference was observed in hypothyroid group treated with thyroxin compared to control group. 
Conclusion: Thyroid hormones have important role on laminin expression and development of lung, since maternal hypothyroidism causes large reduction in alveiolar laminin expression. Therefore changes in maternal thyroid hormone can cause large changes in alveolar development in newborns.