Evaluating the effect of zinc and cobalt nanoparticles on expression of STR, DAT and D4H genes in periwinkle (Cataranthus roseus) suspenssion culture
Volume 7, Issue 4, Spring 2017, Pages 355-364
https://doi.org/10.52547/JCT.7.4.355
M Rezaee, Ramin Hosseini, Behvar Asghari
Abstract Aim: Effects of two nanoparticles were investigated on the expression of STR, DAT and D4H genes in periwinkle.
Material and Methods: For this purpose, concentrations of 0.5, 0.75 and 1 mg per liter of cobalt and zinc oxide nanoparticles were used. Sampling was carried out at 0, 8, 24 and 48 hours after treatment.
Results: Gene expression analysis was performed by the SQ-RT-PCR method. In general, both elicitors influenced gene expression. But, the effects of zinc oxide nanoparticles on the gene expression were more pronounced than cobalt oxide nanoparticles. The highest expression of STR and D4H genes were occurred in 0.5 mg per liter of zinc oxide nanoparticles after 8 hours treatment, while in the case of DAT gene, it occurred in concentration of 1 mg per liter of this nanoparticle. Moreover, Cobalt oxide nanoparticles in most of the studied concentrations and time intervals caused decreases in gene expression.
Conclusion: Both nanoparticles had significant effects on gene expression of vinblastine and vincristine pathways.