Cell and Tissue Journal

Abstract Aim: The effect of  low- frequency electromagnetic waves on motor activity and brain tissue motor area in rats was investigated.
Material and Methods: In this experimental study, 20 rats were randomly divided into four groups. The first group was designated as the control group, and experimental groups received 900, 1800, and 2450 MHz waves, with an intensity of 2, 1, and 20 watts per kilogram, 4 hours per day for one month respectively. Behavioral evaluation of open field test and histomorphometric evaluation of cortical thickness and count of internal pyramidal cells were determined.
Results: The results showed that low- frequency electromagnetic waves significantly reduced the distance traveled and the time spent in the central open field test area of the experimental groups compared to the control group (p ˂ 0.05). The number of neurons in the inner pyramidal layer and the thickness of the cortical thickness of the frontal lobe showed a significant decrease in the experimental groups compared to the control group (p ˂ 0.05).
Conclusion: Low- frequency electromagnetic waves increased anxiety and decreased motor activity in the behavioral tests with a histomorphometric change of frontal cortex in the experimental groups.
 

Abstract Aim: This study aimed to evaluate the histomorphometric and immunohistochemical parameters of the repair of femoral bone defect using bone marrow stromal cells on gelatin – chitosan membrane in adult Albino Wistar rats. Materials and Methods: In this experimental study, sixty male Albino wistar adult rats were equally divided into five groups as follows: Control group that received no treatment after bone defect. Sham group that after bone defect, the culture medium was injected locally at the site of defect. Gelatin-chitosan group that membrane was used into bone defect. Cell group that nonautolog BMSCs were injected locally into defect .Cell-GC group that cell transplantation with chitosan - gelatin membrane were used into the bone defect. Results: The mean area of trabeculae in groups of membranes and cells significantly increased when compared to the control group.The mean number of osteocytes and cells in the bone defect in cell group  significantly increased when compared to the control group.No significant difference were found in chitosan - gelatin and sham groups compared to control group but the mean number of osteocytes significantly decreased in BMSCs with gelatin-chitosan scaffold group compared to control group (p < 0.001) Conclusion: BMSC transplantation and gelatin-chitosan scaffold are effective in repair of bone defect. However, the use of BMSCs with gelatin-chitosan scaffold is not effective in repair of  femoral bone defect.

Abstract Aim: Elaeagnus angustifolia L. is a plant with many therapeutic effects. In this study the effect of aqueous extract of E. angustifolia fruit on Balb/C mouse embryo lens development was investigated.
Material and Methods: Thirty pregnant mice were randomly divided into two equal groups. Control group received drinking water daily and the experimental group received E. angustifolia fruit aqueous extract orally at a dose of 500 mg/kg from zero days to 18th day of pregnancy. After killing all of mice at 18th day gestation, their fetal heads were removed, fixed and processed for histological procedures. Fetal heads were sagitally sectioned at 5 µ thickness and then were stained with hematoxylin-eosin (H&E) and acid fuchsin-light green techniques. Then sections were examined using a light microscope and Motic software.
Results: Results showed that weight and Crown-Rump length mean of fetuses significantly increased in experimental group compared to control. Mean number of fetuses in experimental group was no significant decreased in comparison with control. Means placenta weight and diameter significantly decreased in experimental group compared to control. Also antero-posterior and superior-inferior diameters of lens and lens surface area significantly decreased in experimental group in comparison with control.
Conclusion: These results indicate that using E. angustifolia fruit aqueous extract at 500 mg/kg dose in pregnancy during is caused delaying in mouse embryo lens development.