Study of hypoglycemic effect of Tragopogon graminifolius L. hydroalcoholic leaf's extract in normal and diabetic male rats
Volume 7, Issue 1, Spring 2016, Pages 9-17
https://doi.org/10.52547/JCT.7.1.9
D Z, N M
Abstract Aim: The aim of this study was to evaluate the anti-diabetic effects of Tragopogon graminifolius hydroethanolic leaf's extract (TGE) on blood glucose and insulin level in diabetes male rats. Material and methods: In this experimental study 42 Wistar male rats were randomly divided in 6 groups: control, diabetic (streptozotocin60 mg/kg, i.p), experimental diabetic groups (treatment with TGE , 200, 400 and 800 mg/kg, i.p) and diabetic treatment with metformin (500mg/kg, gavaged) for 10 days. The blood glucose was examined daily by glucometer. At the end of examination blood samples and pancreatic tissue were collected for insulin evaluation and histological study. All data are expressed as mean±SEM and statistical significance was considered at p < strong>Results: Treatment with the 800 mg/kg TGE decreased significantly blood glucose in diabetic rats compared with control group (p < 0.05). In addition, administration of 400 and 800 mg/kg of TGE could significantly decreased plasma glucose levels compared with metformin group. The insulin blood levels increased significantly in treated groups compared with diabetic group (p < 0.05). Conclusion: The TGE has flavonoid and phenolic compositions and its prescription might be decrease blood glucose and increase blood insulin levels in diabetic rats.
Study of Hepatoprotective Effect of Malva neglecta L. Hydroethanolic Leaf Extract in Male Rat Induced with Carbon Tetrachloride
Volume 6, Issue 1, Spring 2015, Pages 31-42
https://doi.org/10.52547/JCT.6.1.31
SF T, N M, A S
Abstract Aim: In this study the hepatoprotectivity effect of Malva neglecta hydroethanolic extract (MHE) were investigated in male rats.
Material and Methods: 35 male rats with 230-250 gr weight were divided in 5 groups: control (taking normal saline,0.5ml/day, IP), sham(taking olive oil, 0.5ml/day,IP),carbon tetrachloride 1:1 with olive oil,0.5ml single dose, IP), treated 1&2 (carbon tetrachloride 1:1 with olive oil,0.5ml single dose and 300mg/Kg and 600mg/Kg MHE /day for 4 days, IP). After the end of examination the blood samples were collected from heart directly and AST, ALT and AP enzymes were analyzed. The microscopic studies of hepatic tissue were done and histological changes observed by (H&E) staining. All data analyzed with ANOVA test. Statistical significance was accepted at p < 0.05.
Results: The serum liver enzymes were increased in rats induced by ccl4 compare to the control group (p < 0.001) and decreased significantly in rats by treated MHE (p < 0.01). Histological finding revealed the necrosis in hepatic tissue. In animals treated with MHE the results showed that the reduction in necrotic cells and increase in hepatocytes regeneration.
Conclusion: Our results showed that the ccl4 has hepatotoxicity effect and it can improve inflammation and necrosis in liver tissue. The MHE have antioxidant and flavonoid that can decrease toxic effect of carbon tetrachloride in liver tissue.