Cell and Tissue Journal

Abstract Aim: In this study, the extract of earthworm (Eisenia foetida) (in solvent: olive oil and vaselline) was applied as a novel healing stimulator on suture wound in goldfish (Carassius auratus).  
Material and Methods: Groups were: negative control (Intact skin), positive control (suture wound with phenytoin), pseudo-control (sham: suture wound with solvent), and experimental (suture wound with 10 mg earthworm extract). A 2 cm longitudinal cut was made from head to tail, and then suture was applied followed by daily treatments. After 14 days, blood samples were collected. Macroscopic and microscopic observations were also done. Meanwhile, the content of lipid peroxidation (LPO/MDA) was measured in fish blood sera.
Results: surface and microscopic observations of wounds revealed a better result for earthworm extract treatment in suture wound healing when compared with phenytoin. MDA level in goldfish blood sera was lesser in earthworm extract-treated groups compared to other groups.
Conclusion: Earthworm extract is a new candidate in aquatic wound healing. Some factors of the extract may enter the blood and ultimately activate the antioxidant systems leading to decrease the content of LPO in fish blood sera.   
 

Abstract Aim: Present study was done to assess the effects of Av gel on some molecular aspects in wound healing process of mouse damaged skin.
Material and Methods: 36 Male mice balb/c were used. Animals were divided into 3 groups; negative control (without wound), sham-operated (wound treated with physiological serum) and experimental (wound treated with Av gel extract). In each group, two equal full-thickness wounds were made on the mice back sides. Experimental group received daily a dose of Av gel extract on the top of wound sites (without bandage) for a period of 16 days. On 8^th and 16^th post wounding day, for the purpose of EGF receptor gene expression evaluation and lipid peroxidation (LPO) , skin and blood serum sampling were done respectively  and data were analyzed by Repeated measures ANOVA at significant level p < 0.05
Results: Av gel extract treatments increased EGF receptor gene expression in the wound area. Wound healing percentage was also increased in experimental groups compared to sham group. It was also found that the amount of LPO end-product in blood sera was reduced to Av treated mice in the end of test.
Conclusion: Av gel extract can induce EGF receptor gene expression in mice damaged skin, and play a pivotal role in wound healing process.