Study of the Effect of Prenatal CSF from H-Tx Rat on Proliferation and Differentiation of the Wistar Rat’s Cortical Progenitor Cells
Volume 5, Issue 1, Summer 2014, Pages 31-37
https://doi.org/10.52547/JCT.5.3.31
M N, Z S
Abstract Aim: The aim of present study was to demonstrate that the obstruction of CSF flow in hydrocephalic Texas (HT-x) rat and the resulting changes in its protein components can cause abnormal cortical development.
Material and Methods: Cortical cells were taken from E18 and E21 Wistar fetuses and cultured for 24 hours in Neurobasal medium. Two groups of experiments were explored. These cells were treated with heated and non-heated CSF collected from E20 and E21fetuses. The cells were cultured for a further 48 hours.At the end of this time, proliferation assays and morphological analysis were performed
Results: Prenatal affected CSF had an inhibitory effect on the proliferation of normal primary fetal cortical cells. Heating the CSF removed this effect.
Conclusion: CSF contains important factors that can stimulate both proliferation and cell differentiation and that some of these are likely to be proteins. Disruption of CSF flow can cause the cortical deficiencies in patients with hydrocephalous.