Investigating the Effects of Hydrocortisone Hormone on Induced Clastogenical Chromosomal Abnormalities on L929 Cell Line Using Micronucleus Assay on Binucleated Cells
Volume 6, Issue 3, Winter 2016, Pages 249-256
https://doi.org/10.52547/JCT.6.3.249
Y S, F H, M M, SH S, H A
Abstract Aim: In this study the effects of stress on induction of structural chromosomal abnormalities on L929 cell line using micronucleus assay on cytokinesis-blocked binucleated cells were investigated in vitro. Material and methods: L929 cells were cultured in DMEM containing 10% FBS. The cells were divided into four groups including; control, 2Gy gamma-irradiated cells, and cells treated with doses of 25, 50 and 100 µg/ml hydrocortisone, and cells co-treated with these three doses of hydrocortisone and irradiation. The treated cells were harvested, stained and chromosome abnormalities were scored using micronucleus assay. Results: Results showed that hydrocortisone did not induce micronuclei as compared to the control group. However, the frequency of micronuclei in cells co-treated with doses of hydrocortisone and irradiation was significantly higher than cells treated only with gamma irradiation (p < 0.05). Conclusion: According to the data of this study, stress hormones are not able to induce any chromosomal abnormalities; however, they are able to increase the cell susceptibility to clastogenic effects of irradiation. Key words: Stress, Hydrocortisone, L929, Gamma ray, Micronucleus