Cell and Tissue Journal

Abstract Aim: The current study aims to utilize varicocele induction in male rat models to evaluate effect and impact of varicocele on DNA integrity.
 
Material and Methods: 66 male Wistar rats were divided into 3 groups: control, sham and varicocele-induced (VI). 2 months post-VI, Sperm parameters, percentage of sperm DNA damage, persistent histone and lipid peroxidation were assessed.
 
Results: Animal weight and their epididymal length shown no significant changes but there were significant differences in testis volume and sperm parameters between VI group versus control and sham groups. Similarly, increased level of DNA damage, persistent histone and lipid peroxidation were evaluated, and compared between varicocele and control group.
 
Conclusion: Based on literature and the result of this study, it is likely that increase oxidative stress following varicocele induction leads to lipide peroxidation, DNA damages and reduced sperm parameters. In the current study, by taking the advantage of homogenous genetic background and high number of animals, we showed that varicocele have damaging effect on DNA and chromatin integrity and should be reversed in human before aiming for pregnancy.
 
 

Abstract Aim: The aim of this study was the effect of zolpidem on oogenesis and FSH, LH hormones of female adult NMRI mouse.
Material and Methods: In this experimental study thirty adult female mice NMRI strain at a mean weight of 30±26 grams were divided into five groups Zolpidem solution was prepared in distilled water at 5, 10, and 20 (mg/kg of body weight) doses, and 0.5 cc injections were doneintraperitoneally every day for 14 days The control group received no injection. The sham group received distilled water and treatment groups of 1, 2, and 3 received doses of 5, 10, and 20 mg/kg The treatment groups were sacrificed one day after the last injection The concentrations of the hormones were measured by the ELISA test The ovary tissue was separated and examined as well as Hematoxylin and eosin painting and the results were evaluated via the tukey-test,(One-way ANOVA) by SPSS program.
Results: The results showed a significant decrease in the mean serum FSH, LH levels in the experimental groups compared to the sham and control (p < 0.05) Also histological studies of sections showed the mean number of graffian, secondary and corpus luteum reduced and the mean number of primordial, growing, atretic and abnormal follicles increased. In addition, a significant reduction in the diameter of secondary, graffian follicles and corpus luteum and a significant increased in the diameter of primary, atretic follicles were observed in the experimental groups, compared to the sham and control groups.
Conclusion: Injection of zolpidem significantly is effective on , oogenesis and concentration of LH,FSH hormones.

Abstract Aim: In this study, we use combination of enzymatic and explant methods, for isolation and culture of ADMSCs.
Materials and Method: Adipose tissues dissected out from the abdominal region of male Wistar rats. Tissues were minced into small pieces (1-2 mm), and then treated with trypsin-EDTA 0.25% for 30 minutes at 37 ̊C in shaker-incubator. Enzymatic treated tissues were centrifuged and floating parts were cultured. Statistical analysis of the cells number was investigated using GraphPad Prismv6 software.
Results: Results showed that ADMSCs isolated with our methods have growth characteristics similar to conventional methods. ADMSCs were maintained up to 10th passage and exhibited a homogeneous population in terms of appearance and morphology. We demonstrated that ADMSCs by these combination methods have mesenchymal characteristic markers (positive for CD90, CD44, CD73, CD105 and negative for CD35, CD45, CD11b).
Conclusion: In the current study, we showed that the combination of enzymatic and explant methods creates a simple, inexpensive and reproducible method for isolation and culture of ADMSCs.
 

Abstract Aim: In the current study, the protective effect of quercetin (as an antioxidant) was investigated on the ovarian tissue and fertility of female rats exposed to cyclophosphamide.
Material and Methods: 24 Wistar rats were divided into four six-membered groups. The first group was treated with cyclophosphamide (30 mg/kg) and the second group was treated with tween solution. The third and fourth groups administered quercetin (50 and 100 mg/kg) with cyclophosphamide (30mg/kg) intraperitonealy for 30 days. The left ovaries of the rats were removed and after sectioning and staining using Hematoxylin-Eosin were examined using light microscopy. In addition, 16 rat were treated in the same way in 4 groups (one male and three female rat in each group), then the mice mating. After birth, growth indexes were studied. All data were analyzed using one-way ANOVA.
Results: Both doses of quercetin significantly increased the number of primordial follicles and diameter of the primary follicles, the number of blood vessels, the number of offspring and their growth indexes, while decreased the number of atretic graffian follicles,. In addition, a significant increase in the diameter of primordial follicles was observed in rats received 100 mg/Kg quercetin compared to rats received only cyclophosphamide.
Conclusion: Quercetin can reduce the side effects of cyclophosphamide on ovarian tissue and improve fertility indexes.
 

Abstract Aim: In this study, the effects of curcumin- coated silver nanoparticles were examined on induction of apoptosis in ovarian cancer A2780 cell.
Material and Methods: Silver nanoparticles coated with curcumin were biosynthesized, then A2780 cells were treated with different concentration of silver nanoparticles. Cytotoxicity effects of silver nanoparticles in A2780 cells were assessed by MTT assay and apoptotic effects of these nanoparticles were examined using DAPI and acridine orange/ propidium iodide staining and caspase 3/9 activation assay. Changes in Bax and Bcl-2 gene expression were analyzed by Real Time PCR.
Results: Findings showed that silver nanoparticles inhibit A2780 cells proliferation in a dose dependent manner. 8 µg/ml 50% decreased cell viability at 24 hours, DAPI and, acridine orange propidium iodide staining represent that percentage of apoptotic cells in the treated groups was increased. The results of Real Time PCR showed that Bax gene expression in cells treated with silver nanoparticles increased, while Bcl-2 gene expression in the treated cells was decreased.
Conclusion: Silver nanoparticles coated with curcumin induce apoptosis in A2780 cancer cells. The use of the nanoparticles should be considered a promising strategy for the treatment of ovarian cancer.