Cell and Tissue Journal

Abstract Aim: In this experimental study effect of grape seed extract on polycystic ovarian syndrome (PCOS) in female Wistar rats, were assessed. Material and Methods: In this experimental study 30 rats were divided into 6 groups including control, the PCOS and four experimental groups. Four experimental groups were treated with intrapritoneal injection of grape seed extract (doses 50, 75, 100, 200 mg/kg for 10 days). Polycystic ovary was induced by subcutaneous injection of 2 mg estradiol valerate in adult rats. Ovaries of all groups were removed, fixed in Bouin’s fixative and 5 µm sections were stained using Hematoxylin–Eosin. Results: Results showed a significant decrease in the number of primordial, primary, preantral, Graafian follicles and corpus luteum in PCOS group with comparison with control (p < 0.05). Measuring granulosa layer and theca thickness, various follicles diameters and their numbers showed much improvement in treated PCOS groups with hydroalcoholic grape seed extract. Fifty and seventy five mg/kg doses were considered as effective doses. Conclusion: According to this study results, it can be concluded that hydroalcoholic grape seed extract can improve the symptoms of polycystic ovarian syndrome.