The effect of Genestein on the proliferation and osteogenic differentiation of Adipose-derived Mesenchymal Stem Cells from Turman hosres
Volume 11, Issue 2, Summer 2020, Pages 154-166
https://doi.org/10.52547/JCT.11.2.154
F Azadi, M Khodaei Motlagh, A Mohammadi Sangcheshmeh, E Seyedjafari
Abstract Aim: In current study was aimed to investigate effects of different concentrations Genestein on osteogenic differentiation and proliferation of adipose driven mesenchymal stem cells (ADMSCs) from Turkman horses.
Material and Methods: In this experimental study, ADMSCs were treated with 10-8,10-7,10-6,10-5 μM concentrations of Genestein with osteogenic medium. The proliferation and cell viability of Genestein was evaluated by MTT test for 7 days after cell culture, and the role of It on osteogenic differentiation was investigated by alizarin red staining, alkaline phosphatase assay and calcium content for 21 days. The data were analyzed with One-way ANOVA.
Results: The results of MTT test showed after treatment of cells by Genestein, it had no toxic effect (p < 0.05). The activity of alkaline phosphatase in 10-5 concentration treated cells was higher than the other which attributed to the end of differentiation process (21th day) (p < 0.05). The results of alizarin red staining and calcium content showed that the maximum level of Genestein has significantly increase of precipitation of mineral ions on extracellular matrix on 14th and 21st days compare other concertation.
Conclusion: According to the results of this study, Genestein promoted differentiation of these cells in to osteoblast cells without any toxicity effect.
Antioxidant effect of carob seedextract (Ceratoniasiliqua L) on quality parameters Farahani ram sperm after freeze-thawing
Volume 11, Issue 1, Summer 2020, Pages 1-12
https://doi.org/10.52547/JCT.11.1.1
M Asgari, M Khodaei Motlagh, M Kazemi Bonchenari, V Vahedi
Abstract Aim: The aim of this study was to determine the effect of Carob seedextract as natural antioxidant on quality cryopreserved Farahani rams breeding sperm.
Material and Methods: In this study semen was collected from five mature ram (weight: 60±5 kg) twice a week using an artificial vagina and the ejaculates were pooled in order to eliminate the individual effect of rams. Different levels of carob seed extracts (0, 0/05, 0/1, 0/15 and 0/2 mL) were added to diluent based tris-egg yolk. After cooling, filling and sealing of the samples, they were frozen and with nitrogen vapor and immersed in liquid nitrogen and were stored until evaluation time. Thereafter and after thawing and incubation for5 min, sperm quality parameters includingmotility, progressive motility, viability (Nigrosine–eosin staining), membrane integrity with Hypoosmotic (Host) and morphology abnormality (Hancock test).
Results: Results indicated that the level of 0/05 mL carob seedextract significantly improved some parameters including motility, viability, plasma membrane and morphology integrity compared to control (p < 0.01).
Conclusion: Results indicated that so added 0/05 of carob seed extract peel to Tris based extender was beneficial in storage in sperm Farahani ram breeding after freeze-thawing.