miR-33a, miR-429 and miR-200c with EMT pathway was investigated in non-small cell lung cancer
Volume 10, Issue 3, Winter 2019, Pages 143-151
https://doi.org/10.52547/JCT.10.3.143
Gh Golshan Rad, Sh Zare Karizi, M Karimipoor
Abstract Aim: In this study, the correlation of miR-33a, miR-429 and miR-200c with EMT pathway was investigated in the non-small cell lung cancer.
Material and methods: Real-Time PCR was used to evaluate the expression levels of the mentioned miRNA in 30 non-small cell lung tumor tissues (66% of cells were in stages I, II and the rest were in stage III). RNA extraction was performed and cDNA synthesized using stem-loop primers. The quantitative evolution of gene expression were performed using specific primers and data was analyzed by 2-∆∆ct
Results: The evaluation of expression changes for miR-33a showed that 11 samples were up-regulated. miR-429 and miR-200c showed an increased expressions in 12 and 18 tumor samples, respectively. There was no significant difference between the levels of expression (P≤ 0.05).
Conclusion: There was no significant change in the level of expression of miR-33a, miR-429 and miR-200c. These results could be due to sample size or the early stage of samples.