Evaluation of heat-shock protein A2 (HSPA2) expression in diabetic male rats after exercise training
Volume 10, Issue 4, Winter 2020, Pages 193-201
https://doi.org/10.52547/JCT.10.4.193
A Saremi, M Parastesh, M Bayat, Z Davood Abadi Farahani
Abstract Aim: The aim of this study was to investigate the effect of aerobic exercise on HSPA2 expression in testes and sperm parameters in diabetic rats.
Material and Methods: Two-month-old male wistar rats were randomly divided into three groups (n=10 in each group): control (C), diabetic (D) and diabetic training (DT). Diabetes was induced with a single intraperitoneal injection of streptozotocin. The DTrats were conditioned to run on a treadmill for the 8-week period. 48 hours after the last session, the testes were removed, and subjected to histological examination, semen analysis and total RNA was isolated from testes to measure by RT-qPCRHSPA2 mRNA expression level. The variance analysis test were applied to analyze the data (p < 0.05).
Results: Findings show that the D group had a decrease in sperm count (P=0.01), motility (P=0.001), as well as increase in abnormal sperm morphology (P=0.001). Furthermore, in the D group, HSPA2 expressionin testicular tissue was significantly reduced (P=0.01). Unlike the D group, in the DT group sperm count (P=0.04) and viability (P=0.02), and HSPA2 expression (P=0.03) were elevated.
Conclusion: In rat testes, diabetes down regulates HSPA2 expression, which is collectively detrimental to semen parameters. Exercise protected effectively against this detrimental effect.