Comparison of Achillea wilhelmsii, Silybum marianumseed, Echinacea purpurea, Adiantum capillus-veneris and apricot kernel extracts effects on the proliferation and apoptosis of breast cancer cells
Volume 11, Issue 1, Summer 2020, Pages 73-86
https://doi.org/10.52547/JCT.11.1.73
L Soltani, M Darbemamieh
Abstract Aim: The aim of this study was to compare the anti-proliferative and apoptotic effects of hydroalcoholic extracts of different herbal medicines (Achillea wilhelmsii, Silybum marianumseed, Echinacea purpurea, Adiantum capillus-venerisand apricot kernel) against breast cancer cells (Mcf-7).
Material and method: For this purpose, the plants were dried and milled, then, soaked in 70% ethanol for 72 hours and their extracts were extracted using a rotary evaporator.Different concentrations of herbal extracts (12.5, 25, 50 and 100 μg/ml) were added to the cancer cell culture medium and their cytotoxicity and apoptotic effects were investigated after 24h by MTT assay and acridine orange - ethidium bromide staining, respectively.Data was analyzed by SPSS software at the significant level of 5%.
Results: Addition of the highestconcentration of all extracts to the culture mediumshowed the most significant anti-proliferative and apoptotic effects (p < 0.05) compared to other concentrations of the same extracts.Also, among the high concentrations (100μg/ml), the highest cell cytotoxicity effects were related to the extracts of Echinacea purpureaand Adiantum capillus-veneris (p < 0.05).
Conclusion: The results of this study indicated that the addition of Echinacea purpurea and Adiantum capillus-venerisextractsto the cell culturesin high concentrationshad the most significant anti proliferative and apoptotic effects on breast cancer cells in comparison with other plant extractsand concentrations.