Induction of Apoptosis in Human Cancer A549 Cells Through Hydroalcoholic Extract of Salvia officinalis
Volume 11, Issue 2, Summer 2020, Pages 100-112
https://doi.org/10.52547/JCT.11.2.100
E Hoveizi, F Pouratar, M Kesmati, A Shahriari
Abstract Aim: The purpose of the current study is to investigate the cytotoxic and oxidative effects of Salvia officinalis hydroalcoholic extract on cancer A549 cells.
Material and Methods: The cells were seeded in plates to investigate concentrations of Salvia officinalis extract and MTT measurement was done to determine IC50 concentration and cell viability on days 1, 3, 5, and 7. Moreover, analyses of superoxide dismutase and catalase enzymes involved in oxidative stress pathway, and AO/EB staining for a qualitative investigation of cell lines has been conducted in order to explore the effects of IC50 concentration on apoptosis induction. Also, Giemsa and DAPI stainings were utilized to explore the morphological changes of cell and nucleus..
Results: IC50 concentration of Salvia extract for A549 cells was determined 5 mg/mL. According to the results, Salvia extract reduced the cell viability of A549 cells. Based on the results, Salvia extract had a significantly greater cytotoxic effect compared to the control sample of A549 cell line. Treatment cells indicated some clear and dose-dependent differences at different times. The results of stainings proved the apoptosis induction in the treatment group. Furthermore, regarding the enzyme expression results the activity of superoxide dismutase and catalase enzymes in the cell groups treated by Salvia extract was significantly increased, compared to the control group.
Conclusion: Consistent with the results of this study, in addition to the anti-oxidative activity Salvia officinalis hydroalcoholic extract revealed significant apoptotic effects on lung cancer cells, considering a time and dose-dependent method.
Behavioral and biochemical evaluation of the effect of magnesium Nano oxide on memory impaired by paradoxical sleep deprivation in male rats
Volume 10, Issue 3, Winter 2019, Pages 168-180
https://doi.org/10.52547/JCT.10.3.168
M Zarei, LA Khajehpour, M Kesmati, H Najafzadeh
Abstract Aim:. The aim of the current study was to evaluate the effect of magnesium Nano Oxide on learning and memory and biochemical changes in the hippocampus of sleep-deprived rats.
Material and Methods: In this study, male rats were used in control groups whereas others used rats were sleep deprived and the rats receiving Magnesium Nano Oxide with doses of 1, 5, and 10 mg/kg. Step-through apparatus was used to evaluate passive avoidance memory. Laboratory kit and glucometer were used for biochemical evaluation.
Results: The results showed that the magnesium Nano oxide in 10 mg/kg/rat can result in increasing efficiency of memory in male rats that experienced memory impaired resulted from sleep deprivation .In addition to afore-mentioned behavioral changes, the investigator found that sleep deprivation decreases the level of Taurine Amino Acid. However, it can increase the blood sugar and cholinesterase enzyme activity. In addition, it didn''''''''t have significant effect on Brain-derived Neurotrophic factor. Upon application of Magnesium Nano oxide, the amount of blood sugar and collinstrase enzyme activity lowered, but the amount of Taurine Amino Acid remained unchanged.
Conclusion: The findings show that in addition to behavioral effects, magnesium Nano oxide causes a change in the amount of biochemical factors affecting memory. That needs to be further investigated.