P Kheradmand; S Vallian; S Esmaeili Mahani
Abstract
- Aim:In this study, the effect of insulin was investigated in induction of drug resistance to doxorubicin in MCF-7 breast cancer cell line. Material and method: MCF-7 cells were pretreated with 10 nM insulin for 48 and 72 hours, respectively. Then, different doses of doxorubicin (1, 5 and 10 μM) ...
Read More
- Aim:In this study, the effect of insulin was investigated in induction of drug resistance to doxorubicin in MCF-7 breast cancer cell line. Material and method: MCF-7 cells were pretreated with 10 nM insulin for 48 and 72 hours, respectively. Then, different doses of doxorubicin (1, 5 and 10 μM) were added for an additional 24 hours and cell viability was determined by MTT assay. Results: Doxorubicin had antitumor effects by reducing the cell survival in a dose-dependent manner. However, the addition of 10 μM doxorubicin in the insulin-treated cells for 72 hours induced significant drug resistance (p < 0.01). Conclusion: The results revealed that insulin could significantly cause the doxorubicin resistance in a time-dependent manner in MCF-7 cells.
