Study of relation on breast cancer and A/T 251 polymorphism of IL-8 gene in Iranian female populations by Tetra Arms PCR
Volume 10, Issue 1, Spring 2019, Pages 12-23
https://doi.org/10.52547/JCT.10.1.12
E Siasi, M gholami, F Ashrafi
Abstract Aim: In this study, relationship between breast cancer and 251A/T polymorphism of IL-8 gene, as the genetic marker, was investigated in the Iranian females population by Tetra arms-PCR.
Materials and methods: In this case-control study, 50 women with breast cancer and 50 healthy women as control group were selected. After extraction of DNA, determination of IL-8 gene 251 A / T polymorphism genotypes was performed by Tetra arms-PCR.
Results: In the control group, frequency of AA, AT, and TT genotypes were 0%, 88% and 12%, respectively and them for case group were 12%, 54% and 34%, respectively. Result showed no significant difference between AA, AT and TT genotypes in the control and case groups (P ≤ 0.05).
Conclusions: In current research, there was no significant difference between A and T alleles in the control and case groups. Therefore, it indicated that there was not association between IL-8 gene 251 A/T polymorphism and breast cancer in the studied Iranian females population.