Cell and Tissue Journal

Abstract Aim: In this study the synergic effect of Chiton lamyi foot alcoholic extract and decellulation rat brain’s tissue on angiogenesis in hen (chick???) chorioalantoic membrane (CAM) was investigated. Material and Methods: In this study, 70 fertilized eggs of Ross strain were divided into 7 treatments groups in ten repetitions: 1. control, 2. DMSO, 3. decellulation rat brain’s tissue, 4. 10mgμl^-1 Chiton lamyi foot alcoholic extract, 5. 20mgμl^-1 Chiton lamyi foot alcoholic extract, 6. decellulation rat brain’s tissue & 10mgμl^-1 Chiton lamyi foot alcoholic extract and 7. decellulation rat brain’s tissue & 20mgμl^-1 Chiton lamyi foot alcoholic extract. All of eggs were placed in the incubator for eight days and treatments induction was done in 8^th day. Then they photographed using photo steriomicroscope in 12^th day. Blood vessels branches length and number were evaluated by Image J software in treatment sites on chorioalantoic membrane. Data were analyzed using ANOVA, SPSS Test (p < /em><0.05). Results: Results showed that Chiton lamyi foot alcoholic extract, 10 and 20mgμl^-1 Chiton lamyi foot alcoholic extracts with decellulation rat brain’s tissue treatments significantly decreased blood vessels branches length and number in comprising with control and DMSO treatments (p < /em>< 0.05). Conclusion: The Synergic usage of Chiton lamyi foot alcoholic extract and decellulation (acellular) rat brain’s tissue has dose inhibitory effect on hen chorioalantoic membrane (CAM) angiogenesis.