Cell and Tissue Journal

Abstract Aim: In the present study, we examined the possibility of astaxanthin usage in the MS model of mice, experimental autoimmune encephalomyelitis (EAE).
Material and methods: EAE disease was induced using MOG35-55 peptide and complete Freund adjuvant (CFU) in female C57BL/6 mice. Mice were divided into control, patient group (MS model, EAE), and prevention group, the group received astaxanthin for 21days before immunization, and astaxanthin treatment was initiated when mice showed the first symptoms of EAE. Each group consisted of 8 mice. The consumption of Astraxanthin was continued for 35 days, and each mouse received astaxanthin about 0.4% of the weight of used pellet (about 400 mg). Cell proliferation was measured by MTT, IL1β, but IL6 levels (proinflammatory cytokines) were measured by ELISA. In addition, the spinal cord tissues were also taken for immunohistopathological evaluations.
Results: Based on the results, the use of astaxanthin produced appropriate clinical and pathological outcomes, lead to decrement of IL1β, and IL6 proinflammatory cytokines (p < 0.0001) production. Immunohistopathologic studies also showed a decrease in leukocyte infiltration in the spinal cord of experimental groups.
Conclusion: Overall, this drug approach can be considered as a useful strategy in the prevention and treatment of multiple sclerosis.
 

Abstract Aim: In the current study, the protective effect of quercetin (as an antioxidant) was investigated on the ovarian tissue and fertility of female rats exposed to cyclophosphamide.
Material and Methods: 24 Wistar rats were divided into four six-membered groups. The first group was treated with cyclophosphamide (30 mg/kg) and the second group was treated with tween solution. The third and fourth groups administered quercetin (50 and 100 mg/kg) with cyclophosphamide (30mg/kg) intraperitonealy for 30 days. The left ovaries of the rats were removed and after sectioning and staining using Hematoxylin-Eosin were examined using light microscopy. In addition, 16 rat were treated in the same way in 4 groups (one male and three female rat in each group), then the mice mating. After birth, growth indexes were studied. All data were analyzed using one-way ANOVA.
Results: Both doses of quercetin significantly increased the number of primordial follicles and diameter of the primary follicles, the number of blood vessels, the number of offspring and their growth indexes, while decreased the number of atretic graffian follicles,. In addition, a significant increase in the diameter of primordial follicles was observed in rats received 100 mg/Kg quercetin compared to rats received only cyclophosphamide.
Conclusion: Quercetin can reduce the side effects of cyclophosphamide on ovarian tissue and improve fertility indexes.