Effect of curcumin on plasma membrane integrity and stress oxidative factors testis and serum in mice treated with cadmium chloride
Volume 9, Issue 1, Summer 2018, Pages 1-11
https://doi.org/10.52547/JCT.9.1.1
sh Chehreii, HR Momeni, Z Atabaki
Abstract Aim: The current research was done to investigate the sperm plasma membrane integrity, oxidative stress factors of sperm and testis in male mice treated with cadmium.
Material and Methods: 24 adult NMRI mice were divided into four groups: 1. Control group 2. Treated with cadmium chloride (5 mg/kg) 3. Treated with Curcumin (100 mg/kg) 4. Treated with curcumin + cadmium chloride. The treatments were performed as a single dose and after 24 hours, epididymal spermatozoa of different groups were used to evaluate the plasma membrane integrity. In addition, the amount of malondialdehyde (MDA) and the anti-oxidant activity of serum and testis were evaluated. Data were analyzed using ANOVA test followed by Turkey’s test (p < 0.05).
Results: findings showed that cadmium chloride caused a significant decrement in plasma membrane integrity compared to the control groups. In addition, cadmium chloride induced a significant increment of MDA in serum and testis and a significant decrement in total antioxidant activity of serum and testis as compared to the group. In curcumin + cadmium chloride- treated group, curcumin could significantly compensate the toxic effect of cadmium on these parameters compared to the cadmium-treated group.
Conclusion: it seems thatcurcumin as a potent antioxidant is able to ameliorate the adverse effects of cadmium chloride on sperm plasma membrane integrity, lipid oxidation and total antioxidant activity of serum and testis.