The effects of mancozeb on the integrity of blood- testis barrier and expression of associated genes
Volume 9, Issue 1, Summer 2018, Pages 56-65
https://doi.org/10.52547/JCT.9.1.56
M Mohammadi-Sardoo, SN Nematollahi-Mahani, M Nabiuni, A Mandegary, T Eslaminejad, B Amirheidari
Abstract Aim: The current study was designed to understand the destructive effects of mancozeb on BTB in an in vitro model.
Material and Methods: Male mice were gavage- administered MZB (250, 500 mg/kg w. w) for 40 consecutive days. Integrity of BTB was evaluated using Evans blue dye. The relative mRNA expression of BTB- associated genes; including N- cadherin, claudin-11 and zonula occludens-1 was investigated by quantitative real-time reverse transcription–polymerase chain reaction in different groups (qRT-PCR).
Results: Concentration of Evans Blue dye in the lumen of seminiferous tubules was increased in mancozeb-treated animals. The mRNA expression levels of N-cadherin, claudin-11 and zonula occludens-1 genes were decreased significantly in the MZB 500 mg/kg group compared with the control group.
Conclusion: According to the obtained data, it could be concluded that mancozeb increase BTB permeability leading to testicular dysfunction by the alteration of BTB-associated genes.