Investigating the anticancer effect of Lippia citriodora leaf alcoholic extract: in suppression of A2780 ovarian cancer cell metastasis via restoration of E-cadherin expression
Volume 10, Issue 1, Spring 2019, Pages 24-33
https://doi.org/10.52547/JCT.10.1.24
E Amini, M Nabiuni, J Baharara, SB Behzad, D Seyfi, F Salek
Abstract Aim: The purpose of this study is to investigate the pro-apoptotic and anti-metastatic potentials of Lippia citriodora alcoholic leaf extract on A2780 ovarian cancer cells and to evaluate the expression of E-cadherin as one of the most important marker in metastasis.
Material and Methods: A2780 ovarian cancer cell line was prepared from Pasteur cell bank and cultured in RPMI1640 medium containing 10% FBS and 1% antibiotic. After cell seeding and treatment with different concentrations of extract (10-400 µg/ml), the cells viability and pro-apoptotic potential were examined by MTT assay and acridine orange/ propodium iodide, respectively. Caspase-3 assay and anti-invasive effect were analyzed by migration assay and assessment of E-cadherin expression, respectively. Then, one way ANOVA test was employed for analysis of quantitative data.
Results: Data indicated that L. citriodora alcoholic extract attenuated ovarian cancer cell viability. Acridine orange/ propodium iodide and caspase-3 assays showed that this extract in IC50 concentration (100 µg/ml) induced apoptosis mainly through caspase dependent pathway in the ovarian cancer cells. Migration assay and RT-PCR exhibited that this extract has anti-invasive capability and by up- regulation of E-cadherin prevents the loss of attachment between cancer cells.
Conclusion: The results revealed that L. citriodora alcoholic extract has apoptosis inducing capacity and ability of restoration E-cadherin expression in A2780 cancer cells, which it able to suppress invasive potential of ovarian cancer cells.