Evaluation of metalloproteinase matrix MMP9 gene expression and effect of silver nanoparticles toward Colon cancer cell line (HT29)
Volume 9, Issue 4, Winter 2019, Pages 344-352
https://doi.org/10.52547/JCT.9.4.344
B Hassani Derakhshandeh, SA Sadat Shandiz, M Abbasi
Abstract Aim: In this study, the toxicity effect of silver nanoparticles on colon cancer (HT29) was measured and evaluate the expression of the matrix metalloproteinase 9 (MMP9) genes.
Material and methods: In this study, the synthesized AgNPs were characterized by the transmission electron microscopy (TEM) analysis. The cytotoxicity effect of silver nanoparticles against the HT29 cell lines was investigated using MTT assay. Also, MMP9 gene expression was measured using real time PCR technique.
Results: The TEM result showed that the fabricated AgNPs were mostly spherical in shape and having an average diameter of 22 nm. The results revealed that AgNPs significantly decreased the viability of cells in dose-and time dependent manner (p < 0.001). Also, there was a significant relationship between the differences in concentrations. MMP9 gene expression levels in HT29 cells were decreased to 0.4897 ± 0.00679 (p < 0.001) compared with the control group.
Conclusion: According to the results, the green fabricated AgNPs can be considered as a promising strategy for the treatment of colon cancer.