Increased expression of Beclin1 and LC3 genes involved in autophagy in non-small cell lung cancer patients
Volume 9, Issue 2, Autumn 2018, Pages 112-122
https://doi.org/10.52547/JCT.9.2.112
M Akbari-Kelishomi, Sh Karizi, M Karimipoor
Abstract Aim: In the present study, the expression of Beclin1 and LC3 genes has been investigated in 30 patients with non-small cell lung cancer.
Material and methods: RNA was extracted from tumor and adjacent normal tissues of 30 patients with non-small cell lung cancer. After synthesis of cDNA, real time-PCR was exploited for quantitative expression of Beclin1 and LC3 genes. Also, the expression of these genes was compared with the clinical and pathological findings of the patients.
Results: the expression levels of Beclin1 (p < 0.0001) and LC3 (p < 0.0001) genes increased significantly. Also, there was a significant correlation between increasing the expression of LC3 with the subtype of tissue (P=0.01).
Conclusion: The increased expression of Beclin-1 and LC3 genes may enhance the process of autophagy and this condition can develop tumorigenesis; however more research is needed to confirm these findings.