Cell and Tissue Journal

Abstract Aim: In this study, the correlation of miR-33a, miR-429 and miR-200c with EMT pathway was investigated in the non-small cell lung cancer.
Material and methods: Real-Time PCR was used to evaluate the expression levels of the mentioned miRNA in 30 non-small cell lung tumor tissues (66% of cells were in stages I, II and the rest were in stage III). RNA extraction was performed and cDNA synthesized using stem-loop primers. The quantitative evolution of gene expression were performed using specific primers and data was analyzed by 2^-∆∆ct
Results: The evaluation of expression changes for miR-33a showed that 11 samples were up-regulated. miR-429 and miR-200c showed an increased expressions in 12 and 18 tumor samples, respectively. There was no significant difference between the levels of expression (P≤ 0.05).
Conclusion: There was no significant change in the level of expression of miR-33a, miR-429 and miR-200c. These results could be due to sample size or the early stage of samples.
 
 

Abstract Aim: In the present study, the expression of Beclin1 and LC3 genes has been investigated in 30 patients with non-small cell lung cancer.
Material and methods: RNA was extracted from tumor and adjacent normal tissues of 30 patients with non-small cell lung cancer. After synthesis of cDNA, real time-PCR was exploited for quantitative expression of Beclin1 and LC3 genes. Also, the expression of these genes was compared with the clinical and pathological findings of the patients.
Results: the expression levels of Beclin1 (p < 0.0001) and LC3 (p < 0.0001) genes increased significantly. Also, there was a significant correlation between increasing the expression of LC3 with the subtype of tissue (P=0.01).
Conclusion: The increased expression of Beclin-1 and LC3 genes may enhance the process of autophagy and this condition can develop tumorigenesis; however more research is needed to confirm these findings.
 

Abstract Aim: In the current study, effect of cerium oxide nano-particles cytotoxicity was investigated on the colon cancer cell line HT29 and evaluation of caspase 3 and 9 apoptosis genes expression.
Material and Methods: In this experimental study, the HT29 cell line was treated with different concentrations of cerium oxide nano-particles, including 0.78, 1.56, 3.12, 6.25, 12.5,25 50 and 100 mg/ml in 24, 48 and 72 hours and the cell viability was determined using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide ) test. Then, the gene expression level of caspase 3 and 9 genes was evaluated by using Real Time PCR in treated HT29 cell line with IC50 value during 24 h. Finally, the apoptosis induction was assessed by flow-cytometry.
Results: The MTT results show that cerium oxide nano-particles had maximum cytotoxicity on HT29 cell line in 25, 50 and 100 mg/ml concentrations in 72 h. Moreover, the Real Time PCR results indicated that the relative expression level of caspase 3 and 9 was up-regulated significantly (2.36±0.76 and 3.4±.95, respectively) in HT29 cell line treated with nano-particle. The flow-cytometry results revealed the 16% apoptosis in HT29 cell line.
Conclusion: Findings showed that the cytotoxicity of cerium oxide was based on time and applied dose. Thus, it appears that this nano-particle could be used in pharmaceutical applications with further studies about selective targeting of cerium oxide nano-particles.