Investigating the effect of Cirsium vulgare hydroethanolic extract on neural stem cells proliferation
Volume 5, Issue 4, Winter 2015, Pages 385-391
https://doi.org/10.52547/JCT.5.4.385
A A, SM Kh, M S, F S, D N, R B
Abstract Aim: In vitro investigation of hydroethanolic extraction of Cirsium vulgare on growth rates of neonate rat neural stem cells. Material and Methods: Neural stem cells were isolated from hippocampus of neonatal rat brain. To determine optimal concentration of Cirsium vulgare extraction, isolated neural stem cells were treated with 200, 400, 600, 800 and 1000 µg/ml for 48 h and then cells proliferation rate were evaluated by MTT assay. In addition Sox2 mRNA expression in neural stem cells was evaluated using quantitative real-time PCR. Results: The results of this study showed that the Cirsium vulgare extract caused significant increase in cell proliferative activity and Sox2 mRNA expression when compared with the control group. Conclusion: with respect to the effect of Cirsium vulgare extract on the proliferation rate of neural stem cells, the use of hydroethanolic extraction of Cirsium vulgare can be used for clinical purposes to treat some of the neurodegenerative disorders such as ischemic stroke and spinal cord injury.