Neuronal differentiation of embryonal carcinoma stem cells in three-dimensional culture system
Volume 7, Issue 3, Winter 2017, Pages 301-311
https://doi.org/10.52547/JCT.7.3.301
F Azizi, H.R Jalil, S.J Moshtaghian, A Doostmohammadi, F Esmaeili
Abstract Aim: The aim of this study was to use carbon nanotube incorporated electrospun chitosan/polyvinyl alcohol (CS/PVA/CNT) to induce neural differentiation of the stem cells for potential neural tissue engineering applications.
Material and Methods: P19 embryonal carcinoma (EC) stem cells were cultured on the CS/PVA/CNT scaffold to induce neuronal differentiation. Cresyl violet staining was used to investigate neuronal phenotype and immunoflourescence technique was carried out to evaluate expression of β-tubulin, a neural specific marker.
Results: Cresyl violet staining confirmed the neuronal morphology of the differentiated cells. These cells were immunoreactive to neuron specific marker, β-tubulin.
Conclusion: the findings suggested the potential use of combined tissue engineering and stem cell therapy to improve deficits in neurodegenerative diseases.