Effect of Glycyrriza glabra L. extract on ODC1 gene expression and cell proliferation in human breast cancer cells
Volume 8, Issue 1, Summer 2017, Pages 42-51
https://doi.org/10.52547/JCT.8.1.42
A Safipour Afshar, F Saeid Nematpour, M Lakzian
Abstract Aim: Product of ODC1 gene is key enzyme in polyamines synthesis that in diverse cancers their content increases. In the present study the effects of root extract of licorice on the ODC1gene expression and cell viability of two human breast cancer cell lines (MCF-7 and MDA-MB-231) and non-cancer cell line (MCF-10A) were evaluated.
Material and Methods: The cell lines were subjected to increasing doses of the extract ranging from 0-200 µg ⁄ml. Cytotoxic effect was assessed using the MTT assay. Expression of ornithine decarboxylase 1 gene was analyzed by Real Time PCR.
Results: In cancerous cell lines mortality increases significantly by a concentration-dependent manner. Moreover, a marked decrease observed in the expression of ODC1 gene in cancer cell lines. In comparison of two cancer cell lines, MDA-MB-231cell line more affected by licorice root extract.
Conclusion: The result of our study highlights the potential influences of Glycyrrhiza glabra extract on ODC1 gene expression in breast cancer cells and its relation to inhibition of cancer cell growth.