Cell and Tissue Journal

Abstract Aim: In this study, the histology effect of caffeine administration was evaluated on retina and PAX6 expression in rat neonates.
Material and Methods: A total of 24 pregnant rats were divided into three groups. Intraperitoneal injection of saline in the control group and also 50 mg/kg and 100 mg/kg caffeine injection from the ninth day of gestation was done. At 20 or 21 embryonic day, neonates on the first and fifth days were euthanized anesthetized and the eyes were isolated and fixed. The neonates were examined for abnormalities. The Retina Histological and morphometric evaluations, eye tissue fluorescence changes, and PAX6 gene expression were investigated by Real time PCR.
Results: The results showed that the effects of maternal caffeine treatment on the retina in neonates are associated with atrophic changes and cell counts of ganglion cell layer, retinal thickness and thickness of the inner plexiform layer, in the animals were treated with caffeine, were significantly reduced (p < 0.05). Also caffeine induced behavioral changes in mothers and tissue fluorescence and significantly increased (P <0.05) the PAX6 gene expression in the groups receiving caffeine.
Conclusion: Administration of caffeine during pregnancy can induce histopathological changes in organogenesis and developing in rat neonate’s retina.
 

Abstract Aim: The aim of the present study was to investigate the effects of royal jelly (RJ) and vitamin C (vit C) against PHZ-induced nephropathy in mice.
Material and Methods: Adult male mice were randomly divided into eight eight-membered groups. The PHZ was administered to four groups of mice at a dose of 60 mg/kg per 48 hours intraperitoneally for 35 days. Three of these groups received vit C (250 mg/kg per day) intraperitoneally, RJ (100 mg/kg per day) orally and vit C+RJ with same doses, four hours before PHZ administration, respectively. A vehicle-treated control group and vit C, RJ and vit C+RJ control groups were also included. Serum and renal tissue samples were collected 24 hours after the last treatment and subjected to biochemical and histological examinations, respectively. The data were analyzed by one-way analysis of variance followed by Duncan test for post hoc comparisons.
Results: The PHZ treatment caused a significant (P < 0.05) increased malondealdehyde concentration in serum, but decreased total antioxidant capacity of it. Moreover, PHZ significantly (p < 0.05) increased luminal diameter of proximal convoluted tubule, while decreased (p < 0.05) their epithelial cells height. Considerably, co-treatment of vit C and RJ improved all changes in the above-mentioned parameters.
Conclusion: The Vit C and RJ can minimize PHZ-induced nephropathy in mouse through oxidative reactions inhibition.