Evaluation of the relationship between Acrosome integrity and some of the cryopreservation traits of Holstein bull sperm
Volume 7, Issue 3, Winter 2017, Pages 251-258
https://doi.org/10.52547/JCT.7.3.251
G Mohammadi, H Mahdion, S Gooraninejad, GH Khadjeh
Abstract Aim: The aim of the present study was to find a simple method for evaluation of acrosomal integrity and evaluation of relationship between acrosomal integrity with motility, viability and reaction to hypoosmolar solution.
Material and Methods: 40 frozen sperm of Holstein bull were purchased and acrosomal integrity, motility, viability and reaction to hypoosmolar solution were examined.
Results: Based on the results, the percentage of acrosomal integrity was 84.52±5.72, motility was 31.52±8.22, alive sperm was 63.57±10.23, abnormal sperm was 21.57±3.23 and reaction to hypoosmolar solution was 41.96±6.68. Results showed the significant positive relationship between acrosomal integrity and motility, and alive sperm and normal sperm (p < 0.01), but no relation between acrosome integrity and reaction to hypoosmolar solution was observed. The results showed a significant positive relationship between abnormal acrosomes and abnormal sperms (p < 0.05), while a significant negative relationship observed between abnormal acrosome with motility and live sperm rate (p < 0.01). No significant relationship was observed between abnormal acrosomes and reaction to hypoosmolar solution (P>0.05). There is a significant relation between acrosome integrity with motility, normal sperm and alive sperm.
Conclusion: Since sperm fertility is related with acrosome integrity, normal sperm, motility and sperm viability and because sperm acrosome during freezing and thawing affected so evaluation of acrosome integrity can be considered as one of the useful examination for assessment of cryopreserved sperm fertility.