Investigating the anticancer effect of Lippiacitriodora leaf alcoholic extract: suppression of A2780 ovarian cancer cell metastasis via restoration of E-cadherin expression
Volume 10, Issue 2, Autumn 2019, Pages 108-117
https://doi.org/10.52547/JCT.10.2.108
F Hashemi Nejad, E Moazamian, M Salouti
Abstract Aim: The aim of this study was purification of magnetosomes of Magnetospirillum gryphiswaldense and investigation cytotoxicity effect of the purified particles of this bacterium on the breast cancer cell lines.
Material and Methods: In this study, the standard strain of bacteria was cultured on DSMZ broth at temperature of 28°C for seven to ten days and incubated under the microaeraphilic conditions. To ensure phenotype tests, the PCR reactions were performed using specific primers (MT1166), associated with scanning electron microscope imaging. The influence of purified magnetosome from Magnetospirillum gryphiswaldense in various concentrations was investigated on the breast cancer cell lines. Kruskalwalis test was performed for statistical analysis.
Results: Gram staining results showed that the bacterium was gram negative spirillum. Electronic micrographs indicated that the magnetosome of this bacterium was 60 nanometer in size. Purified magnetosome from Magnetospirillum gryphiswaldense did not show considerable cytotoxic properties on the breast cancer cell lines. The percentage of living cells against magnetosomes purified from the bacterium Magnetospirillum gryphiswaldense was 85% (p=0.04).
Conclusion: Magnetospirillum gryphiswaldense magnetosomes killed 15% of the cancer cells. The size and structure of magnetosomes of Magnetotactic bacteria are great importance in the elimination of the breast cancer cells.
Cytocidal effects of Bacillus thuringiensis crystal protein on mice breast cancer cell line in in vitro condition
Volume 7, Issue 3, Winter 2017, Pages 243-250
https://doi.org/10.52547/JCT.7.3.243
SH Shekufe Biazar, E Moazamian, N Azarpira
Abstract Aim:In the study, the effects of Bacillus thuringiensis parasporin were examined on mice cancer cell line (4T1).
Material and Methods: Purification of crystal toxins was done from the isolates of B. thuringiensis. Breast cancer cells line 4T1 were treated with activated toxins. Cytopathic effects were photographed. Crystal toxin with the most cytotoxicity was identified by using SDS-PAGE and also molecular method.
Results: From forty seven tested isolates of B. thuringiensis, E8 isolate shown the most cytotoxicity effect on 4T1 cell line. In addition, parasporin-4 was identified by using SDS-PAGE and PCR methods. Parasporin-4 disintegrated 4T1 cell line and it has cytolysin effects.
Conclusion: Our data suggest that, parasporin is cytolysin protein and has a specific site on the cell surface. This crystal protein can induce apoptosis in breast cancer cell line.