Cell and Tissue Journal

Abstract Aim: To study the karyotype and chromosomal characteristics of Garra rufa from Semirom River (Karun River drainage) of Isfahan province. Material and Methods: Seven specimens of Garra rufa was collected by a seine net and transferred live to the laboratory. After squashing of gill and head kidney tissues, the tissue was stained by Giemsa. The mitosis was triggered by PHA, followed by other routine procedures of karyology. Results: From the total 50 mitotic metaphases obtained from 7 individuals, 2n=48 had the highest frequency (52%), consisting of 34 metacentric, 12 submetacentric and 2 acro/telocentric chromosomes. The fundamental number of chromosome arms (NF) was 94. The total lengths of the smallest and largest chromosomes were 6.40 and 1.67 µm, respectively. Conclusions: The diversity in chromosomal number, chromosomal formulae and the fundamental number of chromosome arms (NF), may indicate the presence of an interspecific polymorphism or the presence of several species in this species which need further investigations, especially from a molecular point of view.

Abstract Aim: This study examined the effect of silver nanoparticles (AgNPs) on some hematological tests of Rainbow catfish as a valuable ornamental and food fish species. Material and Methods: 40 fish (average size 12 g) were exposed to different concentrations (1 and 20 µg/L) of AgNPs (average hydrodynamic diameter 54.8 nm) and silver nitrate under constant-renewal condition for 10 days plus a control group (10 fish). At the end of the first and tenth days of exposure, blood samples were taken and conventional hematological tests measured. Results: The mean red blood cell counts (RBC), hematocrit and hemoglobin were increased in 20 µg/L of AgNPs on first day (p < 0.05). There was, however, no differences between treatments at the last day of exposure (P>0.05). In both first and last, there was no changes in the secondary hematological indices (P>0.05). High dose of AgNPs in last day led to increase of white blood cell count (WBC) relative to the control group and first day (p < 0.05). Significant changes in differential white blood cell count (DWBC) were also observed of AgNPs and silver nitrate in both times (p < 0.05). On the tenth day, glucose in both AgNPs treatments was increased (p < 0.05). Conclusion: Significant changes in RBC, hematocrit, hemoglobin, WBC, DWBC and glucose in high dose of AgNPs may indicate toxic effect of AgNPs.

Abstract Aim: Petroleuciscus esfahani, is an important fish species which shows very wide distribution in the Zayandehrood River. As there was no scientific report regarding hematological characteristics of this species, therefore in this study, some major hematological indices of this fish from different stations of the river were analyzed. Material and Methods: Totally, 127 fish were caught from 4 stations along the river including Cheshmeh dimeh, Khersonak, Chamgordan and Pol-e-Safaiyeh and blood was collected from their caudal vein using heparinised syringe. Then some routine hematological indices were measured using standard methods. Results: The mean red blood cell counts, hematocrite and hemoglobin were in the range of 3.02-3.4×10⁶ cell.mm^-3, 28-34.68 % and 9.14-11.69 g.dl^-1 respectively without any significant differences among stations (P>0.05). However, a significant elevation in number of white blood cell (WBC) counts in Chamgordan and Pol-e-Safaiyeh (down-stream) as well as significant changes in differential WBC (DWBC) counts and secondary hematological indices specially in Chamgordan station in comparison to other station were observed  (p < 0.05). Conclusion: The differences in some blood characteristics such as WBC and DWBC may be reflecting the existence of some environmental pollution such as heavy metals in Chamgordan station.