Evaluation the antioxidant effect of crocin on neonate Balb/C mouse spermatogonial stem cells
Volume 7, Issue 3, Winter 2017, Pages 219-229
https://doi.org/10.52547/JCT.7.3.219
A Shokri, J Baharara, E Amini
Abstract Aim: The aim of this study is evaluating the effect of crocin as one of saffron bioactive compounds in mice spermatogenic stem cells.
Material and Methods: Balb/c neonate spermatogenic stem cells were grown in DMEM-F12 medium and were treated with various concentrations of crocin (2.5, 5, 10, 20, 40 µg/ml) for 6 and 12 days. For detecting spermatogenic stem cells, assessment cytotoxicity, recognizing viable cells and antioxidant capacity have been used alkaline phosphatase assay, MTT assay, AO, DAPI staining, and DCF-DA assay, respectively. Statistical analyses, One-way ANOVA, Duncan test(P≤0.05), were conducted using SPSS software .
Results: Crocin in concentrations >20 µg/ml exerted no significant toxicity on mice spermatogenic stem cells in 6 and 12 days treatment. Meanwhile, viability of mice spermatogenic stem cells was reduced under exposure to 20, 40 µg/ml to 21, 24 % in 6 days and 29, 41% in 12 days treatment. Further, evaluations of cell viability by fluorescence microscopy and antioxidant potential by fluorimetery have showed protective and antioxidant effect of crocin after 12 day treatment.
Conclusion: Crocin can be induced protective effect on mice spermatogenic stem cells, which conducted their effect through minimal effects on cell viability and reducing cell death.