Survey of expression of marker genes in spermiogenesis (Protamine1, Acrosin) in induced human spermatogonial stem cells for differentiation into sperm cells
Volume 8, Issue 2, Autumn 2017, Pages 184-195
https://doi.org/10.52547/JCT.8.2.184
Sh Karami, M Maleki
Abstract Aim: The aim of this study is to evaluate the spermatogonial stem cells differentiation into male gametes.
Materia and Methods: Spermatogonial stem cells were cultured in T25 flasks after the enzymatically isolation of the testicular biopsies through azoospermic patients. In the third passage, cells divided into 4 different groups and were treated for 1 to 4weeks under the effect of a medium containing extracts of sheep testes as inducer and then expression of sperm maturation genes: Acrosin and Protamine1 were investigated by using of western blotting technique.
Results: After spermatogonial stem cells treatment by the extracts of sheep testes, variations were seen in cell shape and they convert into sperm- like. Moreover, Acrosin and Protamine1 expression were confirmed.
Conclusion: examination of induced cells showed that Acrosin and Protamin1 were expressed. Since Acrosin and Protamine1 are the major proteins of the spermiogenesis, it could be concluded that these cells had been completed spermatogenesis stage and started the spermiogenesis stage.